REAL TIME RT-PCR ASSAY TO DETECT SARS-COV-2 IN THAILAND

  • Sutchana Tabprasit The Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
  • Krongkan Saipin The Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
  • Kamonwan Siriwatthanakul The Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
  • Min Kramyoo The Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
  • Watcharee Yokanit The Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
  • Wuttikon Rodkvamtook The Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
  • Kunakorn Kana The Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
  • Pramote Imwattana The Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
  • Thanainit Chotanaphuti The Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
Keywords: Real time RT-PCR, COVID-19, SARS-CoV-2

Abstract

The Armed Forces Research Institute of Medical Sciences (AFRIMS) conducts medical research and disease surveillance to develop and evaluate medical products, vaccines and diagnostics to protect Royal Thai Army personnel from infectious diseases. Currently regarding globalized travel, infectious diseases pose a constantly evolving threat, indiscriminately transcending national, regional and even intercontinental boundaries. Since the COVID-19 outbreak, AFRIMS has gained knowledge from diagnostic tests for SARS-CoV-2 using the Centers for Disease Control and Prevention (CDC) as a reference protocol. We set up and developed the molecular diagnosis detection for SARS-CoV-2, Real-time Reverse Transcription Polymerase Chain Reaction (RT-PCR), to analyze the nucleocapsid (N) genome of SARS-CoV-2 which is a standardized method in the laboratory. AFRIMS is certified by the Department of Medical Science, Ministry of Public Health (MOPH) as a COVID-19 laboratory network. We provided COVID-19 screening services to government units and private hospitals in late February 2020. It could be stated that AFRIMS is the first military unit to be certified by the MOPH. Since the COVID-19 pandemic started in Bangkok, 3,172 samples have been tested and 96 samples have been confirmed. Detecting viral RNA not only aids in the diagnosis of illness but also provides epidemiological and surveillance information.

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Real-Time RT-PCR Panel for Detection 2019-Novel Coronavirus by Centers for Disease Control and Prevention (CDC) Effective: 03/30/2020

Published
2021-06-21
How to Cite
1.
Tabprasit S, Saipin K, Siriwatthanakul K, Kramyoo M, Yokanit W, Rodkvamtook W, Kana K, Imwattana P, Chotanaphuti T. REAL TIME RT-PCR ASSAY TO DETECT SARS-COV-2 IN THAILAND. J Southeast Asian Med Res [Internet]. 2021Jun.21 [cited 2021Dec.9];5(1):21-5. Available from: https://www.jseamed.org/index.php/jseamed/article/view/77
Section
Original Articles