TY - JOUR AU - Thunyaharn, Sudaluck AU - Boonsiri, Tanit AU - Visawapoka, Unchalee AU - Santimaleeworagun, Wichai AU - Nitchapanit, Sirachat AU - Noonai, Adisak AU - Suvarnajata, Arunee AU - Kesakomol, Piyanate AU - Rianmanee, Sawayot AU - Sungsirin, Nitchatorn PY - 2022/11/16 Y2 - 2024/03/29 TI - PREVALENCE OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS AND OTHER STAPHYLOCOCCAL NASAL CARRIAGES AMONG HEALTHCARE WORKERS, PHRAMONGKUTKLAO HOSPITAL JF - Journal of Southeast Asian Medical Research JA - J Southeast Asian Med Res VL - 6 IS - 0 SE - Original Articles DO - 10.55374/jseamed.v6i0.122 UR - https://www.jseamed.org/index.php/jseamed/article/view/122 SP - e0122 AB - <p><strong>Background:</strong> Methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) is a group of <em>S. aureus</em> strains containing the SCC<em>mec</em> gene causing beta-lactam antibiotic resistance. MRSA is common in healthcare settings and can cause serious problems.</p><p><strong>Objective:</strong> The study aimed to investigate the prevalence of MRSA nasal colonization among privates of the Medical Private Company, Phramongkutklao Hospital, including antibiotic susceptibility pattern of <em>S. aureus</em> isolates and risk factors of <em>S. aureus</em> nasal carriage.</p><p><strong>Methods:</strong> Nasal swabs were obtained from the anterior nares of 170 privates. Staphylococcal isolates were identified using a catalase test, tube coagulase test and matrix-assisted laser desorption/ ionization time of flight mass spectrometry (MALDI-TOF MS). MRSA detection was screened using cefoxitin disk diffusion and confirmed using the <em>mec</em>A gene detection and SCC<em>mec</em> typing. Antibiotic susceptibility patterns of <em>S. aureus</em> were examined using the disk diffusion method. A questionnaire was collected from the subjects to determine risk factors for <em>S. aureus</em> nasal carriage.</p><p><strong>Results:</strong> Of 170 subjects, 157 (92.35%) revealed staphylococcal positive, yielding 161 staphylococcal isolates. The prevalence of MRSA, methicillin-resistant <em>Staphylococcus epidermidis</em> (MRSE), and methicillin-susceptible <em>Staphylococcus aureus</em> (MSSA) nasal carriage was 0.59, 1.18 and 8.82%, respectively. The MRSA isolate carried <em>mec</em>A revealing SCC<em>mec</em> type II. The MSSA isolates indicated low resistance to tetracycline (13.3%), whereas the MRSA isolate resisted ciprofloxacin, clindamycin, erythromycin, gentamicin, oxacillin and tetracycline. Using multiple logistic regression analysis, a significant risk factor for <em>S. aureus</em> nasal carriage was utensil sharing (adjusted odds ratio=4.41; 95% CI=1.33-14.61).</p><p><strong>Conclusion:</strong> Healthcare-associated MRSA existed among privates of the Medical Private Company. An associated risk factor for acquiring <em>S. aureus</em> was utensil sharing which could be used to help improve prevention and control management among privates.</p> ER -